Nanoengineers at the University of California San Diego have developed microscopic robots that can swim around in the lungs, deliver medication and clear up life-threatening cases of bacterial pneumonia, according to a study published in Nature Materials..
The so-called microrobots safely eliminated pneumonia-causing bacteria in the lungs and resulted in 100% survival. By contrast, untreated mice all died within three days after infection. Made of algae cells whose surfaces are speckled with antibiotic-filled nanoparticles, the algae provide movement, which allows the microrobots to swim around and deliver antibiotics directly to more bacteria in the lungs.
The nanoparticles containing the antibiotics are made of tiny biodegradable polymer spheres that are coated with the cell membranes of neutrophils, which are a type of white blood cell. What’s special about these cell membranes is that they absorb and neutralize inflammatory molecules produced by bacteria and the body’s immune system. This gives the microrobots the ability to reduce harmful inflammation, which in turn makes them more effective at fighting lung infection.
CPAP treatment, which is often used at home to help people with sleep problems, helps to keep the lungs open and makes breathing easier / CREDIT
Lancaster University
The work is a joint effort between the labs of nanoengineering professors Joseph Wang and Liangfang Zhang, both at the UC San Diego Jacobs School of Engineering. Wang is a world leader in the field of micro- and nanorobotics research, while Zhang is a world leader in developing cell-mimicking nanoparticles for treating infections and diseases. Together, they have pioneered the development of tiny drug-delivering robots that can be safely used in live animals to treat bacterial infections in the stomach and blood. Treating bacterial lung infections is the latest in their line of work.
“Our goal is to do targeted drug delivery into more challenging parts of the body, like the lungs. And we want to do it in a way that is safe, easy, biocompatible and long lasting,” said Zhang. “That is what we’ve demonstrated in this work.”
The team used the microrobots to treat mice with an acute and potentially fatal form of pneumonia caused by the bacteria Pseudomonas aeruginosa. This form of pneumonia commonly affects patients who receive mechanical ventilation in the intensive care unit.
The researchers administered the microrobots to the lungs of the mice through a tube inserted in the windpipe. The infections fully cleared up after one week. All mice treated with the microrobots survived past 30 days, while untreated mice died within three days.
Treatment with the microrobots was also more effective than an IV injection of antibiotics into the bloodstream. The latter required a dose of antibiotics that was 3000 times higher than that used in the microrobots to achieve the same effect. For comparison, a dose of microrobots provided 500 nanograms of antibiotics per mouse, while an IV injection provided 1.644 milligrams of antibiotics per mouse.
The team’s approach is so effective because it puts the medication right where it needs to go rather than diffusing it through the rest of the body.
“These results show how targeted drug delivery combined with active movement from the microalgae improves therapeutic efficacy,” said Wang.
And if the thought of putting algae cells in your lungs makes you squeamish, the researchers say that this approach is safe. Next steps include studies to validate the microrobot treatment and scaling it up before testing it in larger animals and eventually, in humans.
